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and impellers by purpose of use

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MRF Reactor

Rotating type mixer for cell culture equipped with an MR210Bio impeller as a standard

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

The MRF Reactor is a BioReactor for laboratory use that aim at actual production and scaling up. In addition to a “Simple shape with excellent cleaning ability” that is important for the actual equipment, the “MR210Bio impeller used exclusively for cell culture” that exhibits excellent mixing and homogenizing performance is provided as a standard, and therefore, similar culture results as during laboratory experiments can be reproduced during scale-up too. Moreover, fluid surface fluctuation (fed-batch culture) is supported, because of which the same mixing performance is exhibited no matter how much the liquid volume. A wide range of liquid volumes from small volumes like 1.5 L to large volumes of 20,000 l can be supported.
With the help of the dedicated controller “S-BOX × 10α II”, changes in various parameters including PI control can be easily performed.

Mixing Impeller Super-mix MR210Bio Used Exclusively for Cell Culture

・Exhibits high mixing performance at low shear
・Simple shape with excellent washing ability
・Completely compatible with fluid surface fluctuation
・The same culture results as in the laboratory can be reproduced during scale-up too.

An environment that is best suited for cell culture is created through excellent mixing performance.

■Flow condition inside culture tank

CFD Simulation Analysis Result

The “MR210Bio” impeller developed exclusively for cell culture produces a high circulating flow that runs from the low-pressure area at the bottom of the blades, where the mixing homogeneity inside the tank is high, toward the top of the tank. As a result of this feature, a high homogeneity and fluidity are obtained even at low power and low rotation, and at the same time, the basic flow pattern does not change even when the fluid surface fluctuates. It is known as an impeller used exclusively for cell culture that adapts to unsteadiness, which was not seen in the past in any impeller, and is suitable for operation under conditions where the fluid surface fluctuates as a result of fed-batch culture. A high circulation performance can be seen from the CFD simulation analysis results.

■Culture example

Comparison of the cell viability in the later stage of the culture by CHO cells

Here we show the cell viability in the later stage of the culture under the batch cell culture condition for the turbine type (flat turbine) and wide paddle type (elephant ear) impellers used in the conventional rotating type BioReactor. It can be confirmed that the MRF reactor maintains a higher cell viability than the other conventional impellers in the later stage of the culture. A characteristic of the MR210Bio that has a low shearing action and high mixing homogeneity performance is the realization of scale-up to a large-size reactor through the combined use of numerical fluid calculation.

▼　MRF Reactor Specifications（Click to open details）

Item		Specifications
Name		MRF Reactor
Model		MRF-3	MRF-8 (10 [%] Dish bottom head)	MRF-10
Temp. control device		Band heater (PID control) with over-temperature protection function (Max. 80 [℃])
Power transmission		Magnet drive (non-sealing type)
Gas supply type		Shirasu porous glass (SPG) membrane type / sintered metal type (selectable)
Performance^*1	Temp. control range	Room temperature + 5 - 20 [℃] (normally set to 37 [℃])
	Temp. accuracy	±0.3 [℃]（37 [℃]）
	Rotational speed range	5 - 200 [min^-1]
Function	Temp. setting	Touch panel input, data output DC1 - 5 [V]
Function	Speed setting	Touch panel input, data output DC0 - 10 [V]
Configuration	Power of band heater	Max. 160 [W]	Max. 480 [W]	Max. 480 [W]
	Power of motor	Max. output 100 [W]
	Mixing impeller	Super-Mix MR210Bio impeller
Culture tank	Dimensions	I.D. 140 x Depth 203 [mm]	I.D. 200 x Depth 330 [mm]	I.D. 200 x Depth 360 [mm]
Culture tank	Culture operation volume	2.4 [L]	6 [L] (10 [%] Dish bottom head)	7 [L]
Usage condition		10 - 35 [℃]
Outer dimensions		W360×D485×H905 [mm]
Weight		Approx. 30 [kg]	Approx. 34 [kg]	Approx. 34 [kg]
Power supply		AC100 [V], 50 /60 [Hz]
*1　The performance is the value under room temperature 20 [℃], power supply AC100 [V], 50 [Hz] and no load condition.

▼　S-BOX×10αⅡ Specifications（Click to open details）

Item	Specifications
Name	Culture controller
Model	S-BOX×10αII
Control	pH, DO (Dissolved oxygen), FL (O₂ flow rate), Pump
Display range/accuracy	pH (Hydrogen ion concentration)		0.00 - 14.00 ［－]		Display accuracy: ±0.5 [%] F.S.
	pH (Hydrogen ion concentration)		0.00 - 14.00 ［－]
	DO (Dissolved oxygen)		0.00 - 20.00 [mg/L]
	DO (Dissolved oxygen)		0.00 - 20.00 [mg/L]
	FL (O₂flow rate)		0.00 - 20.00 [mL/min]
	FL (O₂flow rate)		0.00 - 20.00 [mL/min]
Setting range	pH (Hydrogen ion concentration)		0.00 - 14.00 ［－]
	pH (Hydrogen ion concentration)		0.00 - 14.00 ［－]
	DO (Dissolved oxygen)		0.00 - 10.00 [mg/L]
	DO (Dissolved oxygen)		0.00 - 10.00 [mg/L]
	FL (O₂flow rate)		0.00 - 20.00 [mL/min]
	FL (O₂flow rate)		0.00 - 20.00 [mL/min]
Control type	pH (Hydrogen ion concentration)		ON/OFF control
	pH (Hydrogen ion concentration)		ON/OFF control
	DO (Dissolved oxygen)		O₂addition/subtraction step control
			O₂addition/subtraction step control
			O₂and N₂ON/OFF control
			O₂and N₂ON/OFF control
			O₂PI control (slope set method: TIME, %)
			O₂PI control (slope set method: TIME, %)
	FL (O₂flow rate)		PI control (slope set method: TIME, %)
	FL (O₂flow rate)		PI control (slope set method: TIME, %)
Data output	pH (Hydrogen ion concentration)		With data logger DC0 - 5 [V]		Accuracy: ±0.5 [%] F.S.
	pH (Hydrogen ion concentration)
	DO (Dissolved oxygen)
	DO (Dissolved oxygen)
	FL (O₂flow rate)
	FL (O₂flow rate)
	Rotational speed		DC0 - 10 [V]
	Rotational speed		DC0 - 10 [V]
	Temperature sensor		DC1 - 5 [V]
	Temperature sensor		DC1 - 5 [V]
MTA of outer surface	SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification
Installation	Indoor tabletop type
Outer dimensions/weight	W350×D400×H530 [mm]　・　Approx. 15 [kg]
Usage conditions	Temperature	5 - 45 [℃]	Humidity	20 - 85 [%] RH (No condensation)
Sensors	Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company


	(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd.)


Utilities	Power supply	AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)
	N₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], Connection port φ6 one touch tube fitting
	O₂	Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	CO₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	AIR	Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	*All of N₂, O₂, CO₂, and AIR must be dry and clean gases that do not contain corrosive components, dust, and oil mist.

▼　S-BOX×10 Simple Specifications（Click to open details）

Item	Specifications
Name	Culture controller
Model	S-BOX×10 Simple
Control	pH, DO (Dissolved oxygen)
Display range/accuracy	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]		Display accuracy: ±0.5 [%] F.S.


	DO (Dissolved oxygen)		0.00 - 20.00 [mg/L]


Setting range	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]


	DO (Dissolved oxygen)		0.00 - 10.00 [mg/L]


Control type	pH (Hydrogen ion concentration)		ON/OFF control




	DO (Dissolved oxygen)




Data output	pH (Hydrogen ion concentration)		With data logger DC0 - 5 [V]		Accuracy: ±0.5 [%] F.S.
	pH (Hydrogen ion concentration)
	DO (Dissolved oxygen)



	Rotational speed		DC0 - 10 [V]
	Rotational speed		DC0 - 10 [V]
	Temperature sensor		DC1 - 5 [V]
	Temperature sensor		DC1 - 5 [V]
MTA of outer surface	SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification
Installation	Indoor tabletop type
Outer dimensions/weight	W260×D300×H350 [mm]　・　Approx. 12 [kg]
Usage conditions	Temperature	5 - 45 [℃]	Humidity	20 - 85 [%] RH (No condensation)
Sensors	Optical DO sensor manufactured by Automatic System Research Co., Ltd. + pH sensor manufactured by Mettler Toledo company





Utilities	Power supply	AC100[V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)
	O₂	Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	CO₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	AIR	Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	*All of O₂, CO₂, and AIR must be dry and clean gases that do not contain corrosive components, dust, and oil mist.