The HSF Reactor is a jar fermenter designed for comparatively low-viscosity microbe cultures, plant-based cultures, fungal-based cultures with high viscosity that show non-Newtonian properties, E. coli cultures, etc., in which target cells and bacterial cells have a comparatively strong shear factor, and cultures that require high OTR. It comes with the high-efficiency turbine HS100 and high-discharge axial flow impeller HR100 as a standard. Optimization is possible for scaling up to large-capacity tasks when paired with the S-BOX series controller for culture control.
An optional cooling chiller can be attached, and when using a heat transfer coil type similar to the actual machine, you can achieve conditions that are closer to the actual machine. As a specialty manufacturer of mixing device with the only dedicated mixing technology laboratory in Japan, we can apply final optimizations for actual machine and customize the device according to the physical properties of its contents.

The HS100 turbine is a mixing impeller best suited for gas - liquid mixing. We separately took into consideration the liquid fluidizing action of the impeller and the shearing and destructive actions in a strong discharge field, and examined the flow condition for effectively achieving these actions. The flow is concentrated by using the lifting force of the impeller with low power, and strong shearing and destructive actions are produced by utilizing the pressure gradient and fluctuation in the discharge field. Through this action, high gas absorption performance and the required OTR are achieved. The highest performance capacity is 300 kl.

The HS124ND and HS134ND are turbine impellers having a high gas absorption performance that have been developed to further improve the gas absorption performance. Both the upper and lower blades have an effective discharge action that helps achieve an even higher gas absorption performance and the required OTR. The highest performance capacity is 200 kl.

SATAKE Sparger examines the discharge direction of the gas such that the flowing gas passes through the vicinity of the impeller, which is the shearing field. Moreover, a stationary impeller is installed in the ring, and by increasing the gradient of fluid flow, the shearing and destructive actions are improved. In addition, the stationary impeller also strengthens the discharge action. As a result of these actions, gas can be effectively distributed.

SATAKE takes pride in delivering many culture tanks from small size to large size. Here, we introduce an example of delivery records of large-size cell culture reactors (100 kl to 300 kl class). If you want to order a culture tank as well, the same will be jointly handled by the tank manufacturer working together.
【Motor power】
　・600kW　・480kW　・130kW
　・490kW　・470kW　・110kW
　・485kW　・430kW　・90kW　　etc.

The MRF Reactor is a BioReactor for laboratory use that aim at actual production and scaling up. In addition to a “Simple shape with excellent cleaning ability” that is important for the actual equipment, the “MR210Bio impeller used exclusively for cell culture” that exhibits excellent mixing and homogenizing performance is provided as a standard, and therefore, similar culture results as during laboratory experiments can be reproduced during scale-up too. Moreover, fluid surface fluctuation (fed-batch culture) is supported, because of which the same mixing performance is exhibited no matter how much the liquid volume. A wide range of liquid volumes from small volumes like 1.5 L to large volumes of 20,000 l can be supported.
With the help of the dedicated controller “S-BOX × 10α II”, changes in various parameters including PI control can be easily performed.

The MRF-RB Reactor is a BioReactor developed as a high-performance mixers with equipped with the low-shear, high-efficiency Satake Super-mix^® RB Mixing System that has been used in up to 3,000-ton class biomass reactors. It is a next-generation mixing system that utilizes the boundary layer effect and principles of tornadoes, offering uniform dispersion performance with superb low shearing and low power performance. In continuous culture applications with the basic condition that there is no liquid surface fluctuation, this product is optimal not only for algae cultures, but also animal cell cultures and regenerative medicine. It is extremely easy to scale up, making it optimal for production consideration during the research stage for the actual machine. It is also possible to customize for single use.

The VMF Reactor is a vertical reciprocating motion type next-generation reciprocating mixer for cell culture that combines together excellent blending performance and gentle mixing. Unlike the general rotating type, severe shear stress control is possible. Moreover, a “completely enclosed structure” that does not require a complex rotating-axis seal mechanism is implemented. There is no threat of contamination or leakage, and it has a high level of sterility and cleanliness.
With the help of the dedicated controller “S-BOX × 10αII”, changes in various parameters including PI control can be easily performed. Through customization, up to eight machines in series are supported.

The VerSus Reactor is a new and innovative BioReactor for animal cell culture in which the technology of “SPG membrane sparger*” by which micro-bubbles with extremely high homogeneity can be generated is fused with a VMF reactor. Through an efficient oxygen supply by the SPG membrane sparger, oxygen can be supplied efficiently without putting stress on the animal cells in the culture tank. Also, due to a decline in the DO air flow rate, a foam layer can be prevented. With the help of the dedicated controller “S-BOX × 10α II”, changes in various parameters including PI control can be easily performed.

*SPG membrane sparger has been developed jointly by MIYAZAKI PREFECTURE INDUSTRIAL TECHNOLOGY CENTER and JGC Corporation.

When using iPS cells in 3D floating undifferentiated culture for regenerative medicine, efficient culture medium replacement is important. By stopping the mixing BioReactor for culture medium replacement, cells settle to the bottom of the culture tank having the ability of arbitrarily sticking together on the spot. This process results in uneven cell aggregation (sphere diameter), sometimes adversely affecting induced differentiation efficiency. When induced differentiation is the goal, single cells are discharged along with the culture medium, making it necessary to modify the inside of the culture tank so that they are not affixed to the surface. To satisfy these requirements, clogs must be avoided and culture medium discharge must be stabilized over a long period of time. A control system is an important part of maintaining continuous, precise culture medium replacement. The VMF-WSUB / TCS system has achieved these goals and features specifications that are capable of actual production.

The VMF-50L/200L SUB is a single-use BioReactor for commercial production.
The lineup consists of models from 50 to 200 L (development planned for up to 1,000 L) with tabletop models from 0.5 L to 10 L, which are self-standing from 50 L and up. This is the largest model in the standard VMF reactor series and a commercial production device that is perfectly scalable, offering an unprecedented starting size of 0.5 L. The S-BOX × 200 controller is included as a standard, offering full control over DO, pH, temperature, in-bag pressure, etc., and is equipped with four embedded pumps in a series, a dedicated internal digital data logger, Satake data logger software, and a BioReactor data collection system, etc. It also supports computerized system validation, and documents are provided for qualification confirmation during the design stage, during installation, and during operation. We also provide support for clients who are not accustomed to creating user requested specifications. We guarantee just-in-time supply in small lots of locally-manufactured single-use bags for sterilization validation. We can meet various demands required for commercial production, so please feel free to contact us.

The HiD 4×4 is the world’s first “Single-use mixer for 3D floating cell cultures” used exclusively for iPS cell differentiation induction. A BioReactor that achieves a high-volume production of uniform and homogeneous iPS cells is indispensable not only in regenerative medicine, but also in the “Use of human iPS cells in drug development” that aims at industrialization and commercial production. By promoting joint research and development with excellent research institutes or companies in Japan, we have succeeded in commercialization an iPS cell differentiation induction BioReactor for the first time in the world. 4, 8, 12, and 16 (or even more) such BioReactors can be controlled in a consolidated manner, and these are also best suited for screening. Moreover, we have also taken into consideration the adaptation to ES cells while performing development. With the help of the dedicated controller “S-BOX × 02”, changes in various parameters including PI control, and production control in accordance with the purpose can be performed.